Microdose Precision

TB-500 Microdose Calculator

Sub-milligram precision targets on a standard 100-unit insulin syringe. The optimal BAC water dilution and exact draw units for 250-1000 mcg per-dose research math.

Recommended Dilution
2 mL BAC water in 5 mg vial
Yields 2500 mcg/mL · 25 mcg per insulin unit
Target DoseDraw (units)Volume (mL)
250 mcg10.0 u0.100 mL
500 mcg20.0 u0.200 mL
1000 mcg40.0 u0.400 mL
Open Full TB-500 Calculator → All Microdose Tools
Alternative BAC Volumes
1 mL50 mcg / unit
3 mL17 mcg / unit
Frequently Asked
What BAC water volume gives the cleanest microdose precision for TB-500?
A 2 mL reconstitution of a 5 mg TB-500 vial yields 2500 mcg/mL, which works out to 25 mcg per insulin unit on a 100-unit U-100 syringe. This makes targets like 250 and 500 mcg land on round unit marks instead of fractional half-units, which is the main precision constraint for microgram-scale research dosing.
How many micrograms is one insulin unit of TB-500 at this dilution?
At 5 mg per 2 mL of BAC water, each unit on a 100-unit insulin syringe (1 unit = 0.01 mL) delivers 25 mcg of TB-500. The full draw chart for 250, 500, 1000 mcg targets is calculated on this page.
Can I use a different BAC water volume?
Yes. Larger BAC water volumes produce lower mcg/mL concentrations, which improves precision for very small doses but increases the injection volume. The alternative-volume table on this page shows what happens at 1 mL and 3 mL so the precision tradeoff is explicit. Always verify with the main TB-500 calculator before drawing.
Why does sub-100 mcg precision actually matter?
At the microgram scale, the difference between adjacent insulin unit marks can represent a 50-100% dose change. A single unit error on a target near 100 mcg can double or zero the intended dose. Maintenance phase research protocols often step down from a loading mg dose to sub-mg weekly maintenance.
Does the ASCEND main TB-500 calculator handle these doses too?
The main /calc/tb-500/ page handles the full dose range for TB-500 including the milligram presets. This microdose page is a focused entry point for sub-milligram research targets and links into the main calculator with the dilution pre-filled.

All math is for research reference only. Verify against vial label before drawing. Consult a qualified professional for any clinical application.