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How to reconstitute BPC-157

BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide derived from a gastric protection protein. It is supplied as a lyophilized (freeze-dried) powder and must be dissolved in bacteriostatic water before use. The reconstitution process is straightforward but must be done carefully to preserve the peptide's structure and potency.

TL;DR - Add 2 mL of bacteriostatic water to a 5 mg vial for a concentration of 2.5 mg/mL. Draw to 4 units on a 100-unit insulin syringe for a 100 mcg dose. Swirl gently - never shake. Refrigerate and use within 28 days.
Step-by-step reconstitution
1
Wipe the rubber stopper of both the BPC-157 vial and the bacteriostatic water vial with a fresh alcohol swab. Allow to air-dry for 10 seconds.
2
Draw your chosen volume of bacteriostatic water into a syringe. For a standard 5 mg vial, 2 mL is the most practical starting point (yields 2.5 mg/mL).
3
Insert the needle at an angle so it points toward the inside wall of the BPC-157 vial - not directly at the powder cake. This matters: directing the stream at the powder causes foaming.
4
Push the plunger slowly, letting the water trickle down the glass wall and gently wet the powder from underneath.
5
Remove the needle and gently roll the vial between your palms in slow circles for 20–30 seconds. The solution should become completely clear. BPC-157 dissolves readily and should require minimal agitation.
6
Label the vial with today's date. Refrigerate immediately at 2–8°C.
Tip: If the solution appears slightly cloudy after swirling, place the vial in the refrigerator for 10–15 minutes - the cold temperature aids full dissolution. Do not continue agitating; give it time.
Concentration reference table

The table below shows resulting concentrations for a standard 5 mg BPC-157 vial depending on how much bacteriostatic water you add.

Water Added Concentration 100 mcg dose =
1 mL 5 mg/mL (5,000 mcg/mL) 2 units
2 mL 2.5 mg/mL (2,500 mcg/mL) 4 units
3 mL 1.67 mg/mL (1,670 mcg/mL) 6 units

Units refer to markings on a standard U-100 insulin syringe. Each unit marking = 0.01 mL. The ASCEND calculator handles this math automatically - enter your vial size, water volume, and target dose.

Storage after reconstitution

Once BPC-157 is dissolved in bacteriostatic water, the antimicrobial benzyl alcohol preservative in the diluent slows bacterial contamination but does not stop peptide degradation. Temperature is the primary driver of breakdown.

Store reconstituted BPC-157 in the refrigerator at 2–8°C (36–46°F). At this temperature, the solution is stable for up to 28 days. Keep the vial away from light - wrap in foil or store in a dark compartment if possible. Peptide bonds are susceptible to photodegradation over time.

Lyophilized (unreconstituted) powder stored at room temperature away from moisture is stable for 12–24 months from the manufacturer's date. Some suppliers recommend refrigerating even the dry powder to extend shelf life further.

Warning: Never freeze a reconstituted peptide solution. Ice crystal formation physically ruptures the peptide chain and destroys potency. The lyophilized powder form can be frozen, but once dissolved, it must remain in the 2–8°C refrigerator range only.
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Research References
Sikirić PL et al. - Stable gastric pentadecapeptide BPC 157
Curr Pharm Des 2011 · PMID 19231293
Wang W. - Instability, stabilization of liquid protein pharmaceuticals
Int J Pharm 1999 · PMID 10502313 · Reconstitution & stability basis
ASCEND is a mathematical reference tool for research purposes only. Not for medical use.
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