How long is Glutathione stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

What size insulin syringe should I use for Glutathione?

A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 200 mg Glutathione dose.

RECONSTITUTION GUIDE

How Much BAC Water for Glutathione?

Q: How much BAC water do I add to a 600 mg Glutathione vial?

For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 600 mg Glutathione vial. This yields 300 mg/mL.

Q: Can I use sterile water instead of bacteriostatic water for Glutathione?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.

Quick answer: Add 2 mL of bacteriostatic water to a 600 mg Glutathione vial. This yields a concentration of 300 mg/mL. A standard 200 mg research dose then requires 0.67 mL (66.7 IU on a 100-unit insulin syringe).

200 mg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 200 mg draw volume at common BAC water volumes for a 600 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL	600 mg/mL	0.33 mL	33.3 IU
2 mL Standard	300 mg/mL	0.67 mL	66.7 IU
3 mL	200 mg/mL	1 mL	100 IU
5 mL	120 mg/mL	1.67 mL	166.7 IU

Dose volume at the standard 300 mg/mL concentration

How draw volume changes if you vary the dose at the standard 600 mg + 2 mL reconstitution.

Dose	Volume	IU draw (100u)
100 mg	0.33 mL	33.3 IU
200 mg Std	0.67 mL	66.7 IU
300 mg	1 mL	100 IU
400 mg	1.33 mL	133.3 IU
600 mg	2 mL	200 IU

Reconstitution steps

Wipe the top of both the Glutathione vial and the bacteriostatic water vial with alcohol swabs.
Draw 2 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the Glutathione vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

Glutathione 600 mg lyophilized vial
2 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Other common Glutathione vial sizes

Standard reconstitution volumes and 200 mg draw amounts for 200 mg, 400 mg, and 1000 mg vials.

Vial size	BAC water	Concentration	200 mg draw
200 mg	1 mL	200 mg/mL	1 mL (100 IU)
400 mg	2 mL	200 mg/mL	1 mL (100 IU)
600 mg Most common	2 mL	300 mg/mL	0.67 mL (66.7 IU)
1000 mg	4 mL	250 mg/mL	0.8 mL (80 IU)

About Glutathione reconstitution

Glutathione is a tripeptide antioxidant (gamma-L-glutamyl-L-cysteinylglycine) supplied as a white lyophilized powder. It dissolves readily in bacteriostatic water with minimal mixing -- gentle swirling for 30 to 60 seconds is typically sufficient. Unlike some peptides, glutathione does not require warming or prolonged dissolution time.

Research protocols most commonly use subcutaneous (SubQ) administration for glutathione reconstituted with bacteriostatic water. The 600 mg vial reconstituted with 2 mL is the standard because it produces a manageable draw volume for the 200 mg reference dose. Common research doses range from 100 mg to 600 mg per administration depending on the protocol.

Glutathione is highly sensitive to oxidation and light. Once reconstituted, store at 2–8°C in an opaque container and use within 14 days for best stability -- shorter than many peptides due to its susceptibility to oxidative degradation. A yellow or golden tint in the reconstituted solution indicates oxidation; discard that vial.

Last reviewed: June 2026

Open Glutathione Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 600 mg Glutathione vial?

Add 2 mL of bacteriostatic water to a 600 mg Glutathione vial. This yields 300 mg/mL. A 200 mg dose then requires 0.67 mL (66.7 IU on a 100-unit insulin syringe).

How much BAC water do I add to a 200 mg Glutathione vial?

Add 1 mL of bacteriostatic water to a 200 mg Glutathione vial. This yields 200 mg/mL. A 200 mg dose then draws to 100 IU (1 mL) on a 100-unit syringe.

How much BAC water do I add to a 400 mg Glutathione vial?

Add 2 mL of bacteriostatic water to a 400 mg Glutathione vial. This yields 200 mg/mL. A 200 mg dose then draws to 100 IU (1 mL) on a 100-unit syringe.

How much BAC water do I add to a 1000 mg Glutathione vial?

Add 4 mL of bacteriostatic water to a 1000 mg Glutathione vial. This yields 250 mg/mL. A 200 mg dose then requires 0.8 mL (80 IU on a 100-unit syringe).

What concentration does 2 mL BAC water give for 600 mg Glutathione?

A 600 mg Glutathione vial reconstituted with 2 mL of bacteriostatic water yields 300 mg/mL. A 200 mg dose then requires 0.67 mL (66.7 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for Glutathione?

Bacteriostatic water is preferred for multi-dose vials because the 0.9% benzyl alcohol inhibits bacterial growth. For glutathione specifically, use the vial within 14 days of reconstitution due to oxidative sensitivity. Sterile water can be used for single-use preparations only.

How long is Glutathione stable once reconstituted?

Reconstituted glutathione is stable for approximately 14 days at 2–8°C in an opaque container -- shorter than most peptides due to its oxidative sensitivity. Discard any vial showing a yellow or golden tint, which indicates oxidation.

How do I know if my Glutathione has gone bad?

A yellow or golden tint in the solution indicates oxidation -- discard the vial. Fresh reconstituted glutathione is clear and colorless. Cloudiness or visible particulates also indicate degradation.

What size syringe should I use for a 200 mg Glutathione dose?

At the standard 300 mg/mL concentration (600 mg vial + 2 mL BAC water), a 200 mg dose draws to 0.67 mL (66.7 IU). A 1 mL syringe marked in 0.01 mL increments provides the best precision for this volume.

What is the difference between Glutathione SubQ and IV administration?

The reconstitution process is identical for both routes. For SubQ, the 600 mg + 2 mL standard produces an appropriate concentration. For IV push, some protocols further dilute the reconstituted solution in normal saline. This guide covers reconstitution only; administration method is protocol-dependent.

For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org