ASCEND Peptide Calculator
About & Data Integrity
Research-Grade Precision

Built for researchers who
demand accuracy.

ASCEND is a peptide dosing calculator. Where available, presets are sourced directly from published clinical trial data, not forum posts or guesswork. Independent lab verification confirms what's actually in each vial.

Clinical-Standard Dosing
Where available, presets reflect peer-reviewed Phase 1/2/3 trial protocols. Titration schedules, reconstitution volumes, and dose ranges are sourced from original research publications where possible, not anecdote.
Third-Party Lab Verification
Our Certificate of Analysis database is built on independent laboratory testing. Where available, CoA entries are traceable to a batch number and third-party analyst report, not supplier self-certification.
Privacy by Design
Profiles are encrypted end-to-end via Supabase. No data is sold or shared with third parties. You own your protocol history; export or delete it anytime.
By the numbers
83
Peptide Presets
100%
Free to Use
3rd
Party Verified
Methodology

Where our data comes from

Where available, dosing presets are built from primary literature: published Phase 2 and Phase 3 trial protocols, FDA pharmacology reviews, and peer-reviewed pharmacokinetic studies. When multiple sources conflict, we favor the most conservative, well-powered study.

Reconstitution volumes are calculated to maximize measurement precision on standard U-100 insulin syringes. We flag doses that require splitting across multiple vials.

Phase 2 / 3 Clinical Trials
NEJM, JAMA, Diabetes Care, and other peer-reviewed publications
Independent CoA Analysis
HPLC purity testing, mass spectrometry identity confirmation
Pharmacokinetic Data
Half-life, bioavailability, and dosing interval derived from PK studies
Lab Verification

Reviewed & Verified By

Certificate of Analysis data on ASCEND is independently analyzed and verified by credentialed laboratory scientists at BTLabs and Janoshik: real humans with real credentials, not AI-generated summaries. These are examples of the analysts and scientists involved in verification - the full team spans multiple institutions.

BTLabs - Independent Testing Laboratory
Verified by Independent Analysts
AC
Andrea Castro, AS
Scientist-II · BTLabs, West Palm Beach FL
VZ
Venus Zhang, AS
Scientist-II · BTLabs, West Palm Beach FL
BTLabs performs FTIR identification, HPLC purity assay, and potency analysis on submitted samples. Analysts are credentialed Applied Scientists operating under USP testing protocols.
Janoshik - Analytical Testing Services
Why We Built This

The ASCEND Mission

Peptide reconstitution math is deceptively simple to get wrong. A researcher mixing a 5mg vial with 2mL bacteriostatic water is working in milligrams - but the moment they draw into a U-100 insulin syringe, they're reading in units. That unit-to-volume translation is where errors happen.

ASCEND exists to be the single source of truth for that calculation. Enter your vial size, water volume, and target dose - the app returns the exact syringe marking to draw to, no conversion table needed. For blended vials (GHK-Cu + BPC-157 + TB-500, for example), it handles each peptide's ratio independently so the math stays correct regardless of stack complexity.

How the Math Works

Precision Standards

All syringe calculations are based on the U-100 insulin syringe standard: 1mL = 100 units. This is the industry-universal scale used in peptide research - a 10-unit mark equals 0.10mL regardless of the syringe brand.

Concentration is derived from the vial's labeled peptide mass divided by the volume of reconstitution solution added. Dose-to-volume conversion then divides the target dose by that concentration. For multi-peptide blends, each component's ratio is calculated independently against the total vial mass before applying the same pipeline - ensuring accuracy when peptides share a single vial at different weights.

Evidence Base

Clinical Data Sources

The mathematical logic and dosing presets within ASCEND are derived from clinical trial data and peer-reviewed literature, including:

Wilding JPH, Batterham RL, et al. Once-Weekly Semaglutide in Adults with Overweight or Obesity (STEP 1 Trial). N Engl J Med. 2021;384:989-1002.
Jastreboff AM, Aronne LJ, et al. Tirzepatide Once Weekly for the Treatment of Obesity (SURMOUNT-1). N Engl J Med. 2022;387:205-216.
Jastreboff AM, Kaplan LM, et al. Triple-Hormone-Receptor Agonist Retatrutide for Obesity: A Phase 2 Trial. N Engl J Med. 2023;389:514-526.
Goldstein AL, Hannappel E, Kleinman HK. Thymosin beta4: actin-sequestering protein moonlights to repair injured tissues. Trends Mol Med. 2005;11(9):421-429.
Pickart L, Vasquez-Soltero JM, Margolina A. GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Rejuvenation. Biomed Res Int. 2015;2015:648108.

All clinical presets are cross-referenced with Phase 2 and Phase 3 trial data. While these presets represent the protocols used in clinical settings, ASCEND is designed solely for mathematical calculation and research organization.

Important

Research Disclaimer

WARNING: ASCEND IS A MATHEMATICAL TOOL FOR RESEARCH PURPOSES ONLY.

It is not a medical device and does not provide medical advice, dosing recommendations, or treatment plans. Calculations are provided as-is for informational and educational use. Always verify outputs independently and consult a qualified healthcare professional before beginning any research protocol. Peptide research compounds are not FDA-approved for human therapeutic use in most jurisdictions.

Transparency

Privacy & Open Source

All calculations run locally on your device. ASCEND is built with vanilla JavaScript - there is no server-side computation, no data sent to external APIs for your inputs, and no tracking of what you calculate. Your vial sizes, doses, and protocol details never leave your browser.

Account data (saved protocols, preferences) is stored in Supabase with row-level security - only your account can read your records. You can use every calculator on this site without signing in.

Get in touch

Contact & Feedback

Found an error in a dosing preset? Have a CoA to submit? Want to suggest a peptide? We read every message.

[email protected]
We respond within 24 hours
Primary References
  1. Wilding JPH et al. Once-Weekly Semaglutide in Adults with Overweight or Obesity. NEJM 2021.
  2. Jastreboff AM et al. Tirzepatide Once Weekly for the Treatment of Obesity. NEJM 2022.
  3. Jastreboff AM et al. Triple–Hormone-Receptor Agonist Retatrutide for Obesity. NEJM 2023.
  4. Sikiric P et al. Stable Gastric Pentadecapeptide BPC 157 (Body Protection Compound). Curr Pharm Des 2018.
  5. Pickart L et al. GHK Peptide as a Natural Modulator of Multiple Cellular Pathways. Biomed Res Int 2015.
  6. Baar MP et al. Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis. Cell 2017.
  7. Lee C et al. The Mitochondrial-Derived Peptide MOTS-c Promotes Metabolic Homeostasis. Cell Metab 2015.
  8. Falutz J et al. Effects of Tesamorelin on Visceral Fat and Liver Fat in HIV Patients. NEJM 2010.
All clinical dosing presets are derived from peer-reviewed literature and Phase 2/3 trial data where available. Research-use only.