Metabolic & Weight

Semaglutide Dosing & Reconstitution Calculator

GLP-1 receptor agonist for blood sugar control and significant weight reduction.

TL;DR — Semaglutide is a metabolic & weight peptide with a typical starting dose of 0.25 mg. For a 5 mg vial, add 1 mL bacteriostatic water to yield 5 mg/mL.
0.25 mg
Start Dose
5 mg
Vial
1 mL
BAC Water
5
mg/mL
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Frequently Asked
How do I reconstitute Semaglutide?
Add 1 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 5 mg/mL.
What is the recommended dose of Semaglutide?
A typical research dose is 0.25 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a Semaglutide vial?
A 5 mg vial reconstituted with 1 mL BAC water yields approximately 20 doses at 0.25 mg each.
Peptide Intelligence
What is the half-life of Semaglutide?
Semaglutide has a half-life of approximately 7 days, achieved via albumin-binding fatty acid modification. This allows once-weekly subcutaneous administration with stable steady-state plasma concentrations.
Should Semaglutide be injected subcutaneously or intramuscularly?
Semaglutide is administered subcutaneously only. Preferred sites include the abdomen, outer thigh, and upper arm. Intramuscular injection is not recommended and may alter absorption kinetics.
What is the typical onset time for Semaglutide weight loss effects?
Appetite suppression typically begins within the first week at starting doses. Clinically meaningful weight loss (5%+ body weight) is generally observed by weeks 12–16. Full efficacy develops over 52+ weeks as doses titrate upward.
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Primary Sources
STEP 1 (NEJM 2021) SUSTAIN-6 Cardiovascular ClinicalTrials NCT04788615
Data last reviewed 2026-04-19 · Methodology →
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