Protocol
Longevity and Anti-Aging
NAD+ Peptide Research Protocol [2026]
Restore NAD+ levels for mitochondrial function, sirtuin activation, and cellular energy research.
Compounds
NAD+
Dose: 100 mg
Freq: Once daily for 5 to 10 days
Recon: 500mg + 5mL BAC
Conc: 100 mg/mL
Draw: 10 units (U-100)
Calculate NAD+ dose
Protocol Phases
Loading Cycle
5 to 10 consecutive days
100 mg to 200 mg SubQ or IV once daily. IV produces faster systemic effects but SubQ is more practical for research use. Lower doses are better tolerated; IV at 200 mg can cause significant flushing and nausea.
Maintenance
Monthly to quarterly
Repeat loading cycle every 4 to 12 weeks depending on protocol. Some research uses 50 mg to 100 mg weekly as ongoing maintenance.
FAQ
What is NAD+ and why does it matter?
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme essential for mitochondrial energy production and sirtuin activity. NAD+ levels decline 50% or more from youth to middle age. Restoring NAD+ is researched for improvements in cellular energy, DNA repair, metabolism, and longevity-related sirtuin signaling.
What is the difference between subcutaneous and IV NAD+?
IV NAD+ delivers the compound directly into circulation for immediate systemic availability. SubQ requires absorption through tissue and has slower onset. IV is associated with stronger flushing and GI effects, particularly at doses above 100 mg, often requiring slow drip protocols. SubQ is significantly more convenient and produces gradual steady delivery.
Can NAD+ be stacked with other longevity peptides?
Yes. Common stacks include NAD+ with Epithalon (telomere support), NAD+ with GHK-Cu (skin and matrix), and NAD+ with BPC-157 (gut and tissue). These are typically run sequentially in separate cycles rather than simultaneously.
How long until I notice effects from NAD+?
Some research subjects report improved energy and mental clarity within the first 2 to 3 days of a loading cycle. More systemic effects on mitochondrial function and metabolism may take multiple cycles to assess. Individual variability is high.
Research Notes
- SubQ injection is the most practical delivery route for research use.
- IV administration at higher doses requires slow infusion to manage flushing and nausea.
- Store lyophilized powder at room temperature away from light. Refrigerate after reconstitution.
Related Protocols
For research use only. Not medical advice.