Metabolic & Longevity
NAD+ Dosing & Reconstitution Calculator
NAD+ (Nicotinamide Adenine Dinucleotide) — coenzyme critical for cellular energy metabolism. 500 mg vial. Titrate: 50 mg week 1 → 75 mg week 2 → 100 mg week 3+. Highly unstable after reconstitution.
TL;DR — NAD+ is extremely unstable once reconstituted — prepare fresh doses and use within 24–48 hours. Titrate slowly to minimize flushing: 50 mg week 1, 75 mg week 2, 100 mg week 3+. Standard vial: 500 mg in 3 mL.
⚠ Note — ⚠ Highly unstable after reconstitution. Use within 24–48 hours. Titrate: 50mg wk1 → 75mg wk2 → 100mg wk3+
100 mg
Target Dose
500 mg
Vial
3 mL
BAC Water
~167
mg/mL
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Frequently Asked
How do I reconstitute NAD+?
Add 3 mL of bacteriostatic water to a 500 mg NAD+ vial, injecting slowly down the glass wall. Swirl gently — never shake. This yields approximately 167 mg/mL. Use within 24–48 hours — NAD+ degrades rapidly in solution.
Why must NAD+ be used within 24–48 hours?
NAD+ in aqueous solution is highly susceptible to hydrolysis and oxidation. Unlike most peptides, it cannot be stored reconstituted for days or weeks. Prepare a fresh dose immediately before each administration. Never pre-mix and store.
What is the titration protocol for NAD+?
Start at 50 mg in week 1 to assess tolerance (flushing, nausea, chest discomfort are common at higher doses). Increase to 75 mg in week 2 if tolerated. Target dose of 100 mg in week 3 and beyond. Some protocols go higher but always titrate slowly.
How many doses per NAD+ vial?
A 500 mg vial at 167 mg/mL yields 5 doses at 100 mg each, 6–7 doses at 75 mg, or 10 doses at 50 mg. Given the 24–48 hr reconstitution window, reconstitute only what is needed immediately.
Peptide Intelligence
What does NAD+ do in cells?
NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme in hundreds of redox reactions, essential for ATP production via the electron transport chain. It is also a substrate for sirtuins (SIRT1-7) and PARPs, linking it to DNA repair, circadian rhythm regulation, and aging pathways.
IV vs subcutaneous NAD+ — which is better?
IV NAD+ delivers the coenzyme directly into systemic circulation and is associated with faster effects but also more pronounced side effects (flushing, nausea). Subcutaneous administration is slower and generally better tolerated, with research suggesting comparable bioavailability over a longer time window.
How does NAD+ compare to NMN and NR?
NMN and NR are NAD+ precursors that must be converted intracellularly to NAD+. Direct NAD+ supplementation bypasses this conversion but may have lower cellular uptake vs precursors. The relative efficacy is debated; injectable NAD+ avoids first-pass metabolism that limits oral bioavailability.
Also Explore
Primary Sources
Rajman et al. — NAD+ Therapeutics (2018)
Verdin — NAD+ Aging Review (2015)
Data last reviewed 2026-04-20 · Methodology →
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