Longevity
MOTS-C Dosing & Reconstitution Calculator
Mitochondrial-derived peptide enhancing metabolic homeostasis and exercise capacity.
TL;DR - MOTS-C is a longevity peptide with a typical starting dose of 10 mg. For a 10 mg vial, add 2 mL bacteriostatic water to yield 5 mg/mL.
10 mg
Start Dose
10 mg
Vial
2 mL
BAC Water
5
mg/mL
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Frequently Asked
How do I reconstitute MOTS-C?
Add 2 mL of bacteriostatic water to a 10 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 5 mg/mL.
What is the recommended dose of MOTS-C?
A typical research dose is 10 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a MOTS-C vial?
A 10 mg vial reconstituted with 2 mL BAC water yields approximately 1 doses at 10 mg each.
How many IU is 10 mg of MOTS-C on a syringe?
With a 10 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 5 mg/mL. For a 10 mg dose: Volume = 10 mg ÷ 5 mg/mL = 2 mL. Draw to the 200 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a MOTS-C dosage calculator do?
A MOTS-C dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (10 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the MOTS-C reconstitution calculator formula?
Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 10 mg vial with 2 mL BAC water: concentration = 5 mg/mL. For a 10 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is MOTS-C and where does it come from?
MOTS-C (Mitochondrial Open Reading Frame of the 12S rRNA-c) is a 16-amino acid peptide encoded within the mitochondrial genome's 12S rRNA gene. It is the first mitochondria-derived peptide shown to have systemic regulatory effects, acting as a mitokine communicating metabolic stress to peripheral tissues.
What metabolic effects does MOTS-C produce?
MOTS-C activates AMPK, improves insulin sensitivity, enhances glucose uptake in skeletal muscle, and reduces lipid accumulation. In rodent studies it prevents diet-induced obesity and metabolic syndrome. Circulating MOTS-C levels decline with age, making it a longevity research target.
What is the half-life of MOTS-C?
MOTS-C has a relatively short plasma half-life estimated at a few hours, necessitating daily or multiple-times-weekly subcutaneous injections in research protocols. Exercise is known to naturally increase endogenous MOTS-C secretion, which partially explains exercise's metabolic benefits.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for MOTS-C?
10 mg vial reconstitution recipe + concentration table
MOTS-C on Insulin Syringe
10 mg draw on 30u / 50u / 100u syringes
MOTS-C + Humanin Stack
Mitochondrial-derived peptide stack
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Primary Sources
Mitochondrial Peptide (Cell Metab 2015)
Data last reviewed 2026-06-03 · Methodology →
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