Longevity
SS-31 Dosing & Reconstitution Calculator
Mitochondria-targeted peptide (Elamipretide) protecting against oxidative stress.
TL;DR - SS-31 is a longevity peptide with a typical starting dose of 10 mg. For a 50 mg vial, add 2 mL bacteriostatic water to yield 25 mg/mL.
10 mg
Start Dose
50 mg
Vial
2 mL
BAC Water
25
mg/mL
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Frequently Asked
How do I reconstitute SS-31?
Add 2 mL of bacteriostatic water to a 50 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 25 mg/mL.
What is the recommended dose of SS-31?
A typical research dose is 10 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a SS-31 vial?
A 50 mg vial reconstituted with 2 mL BAC water yields approximately 5 doses at 10 mg each.
How many IU is 10 mg of SS-31 on a syringe?
With a 50 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 25 mg/mL. For a 10 mg dose: Volume = 10 mg ÷ 25 mg/mL = 0.4 mL. Draw to the 40 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a SS-31 dosage calculator do?
A SS-31 dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (50 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the SS-31 reconstitution calculator formula?
Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 50 mg vial with 2 mL BAC water: concentration = 25 mg/mL. For a 10 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is SS-31 and how does it work?
SS-31 (D-Arg-dimethylTyr-Lys-Phe-NH2) is a mitochondria-targeting tetrapeptide that concentrates in the inner mitochondrial membrane by binding cardiolipin. It prevents cytochrome c release, reduces ROS production, and restores mitochondrial cristae architecture in damaged cells.
What is the half-life of SS-31?
SS-31 has a plasma half-life of approximately 2–3 hours after subcutaneous injection, but its tissue half-life in mitochondria-rich tissues such as heart, kidney, and brain may be significantly longer. Daily subcutaneous injections are used in most research protocols.
What diseases is SS-31 being clinically researched for?
Clinical trials have studied SS-31/Elamipretide for Barth syndrome, heart failure, dry age-related macular degeneration, primary mitochondrial myopathy, and renal ischemia-reperfusion injury. Multiple Phase 2 trials have demonstrated safety and preliminary efficacy signals.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for SS-31?
50 mg vial reconstitution recipe + concentration table
SS-31 on Insulin Syringe
10 mg draw on 30u / 50u / 100u syringes
SS-31 + NAD+ Stack
Mitochondrial membrane protection + redox cofactor
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Primary Sources
Elamipretide Review (Front Physiol 2020)
ClinicalTrials NCT04063332
Data last reviewed 2026-06-03 · Methodology →
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