RECONSTITUTION GUIDE

How Much BAC Water for NAD+?

Q: How long is NAD+ stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

Q: What size insulin syringe should I use for NAD+?

For a 50 mg dose (30 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.

Quick answer: Add 3 mL of bacteriostatic water to a 500 mg NAD+ vial. This yields a concentration of 166.67 mg/mL. A standard 50 mg research dose then requires 0.3 mL (30 IU on a 100-unit insulin syringe).

50 mg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 50 mg draw volume at common BAC water volumes for a 500 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL	500 mg/mL	0.1 mL	10 IU
2 mL	250 mg/mL	0.2 mL	20 IU
3 mL Standard	166.67 mg/mL	0.3 mL	30 IU
5 mL	100 mg/mL	0.5 mL	50 IU

Dose volume at the standard 166.67 mg/mL concentration

How draw volume changes if you vary the dose at the standard 500 mg + 3 mL reconstitution.

Dose	Volume	IU draw (100u)
25 mg	0.15 mL	15 IU
50 mg Std	0.3 mL	30 IU
75 mg	0.45 mL	45 IU
100 mg	0.6 mL	60 IU
150 mg	0.9 mL	90 IU

Reconstitution steps

Wipe the top of both the NAD+ vial and the bacteriostatic water vial with alcohol swabs.
Draw 3 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the NAD+ vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

NAD+ 500 mg lyophilized vial
3 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Other common NAD+ vial sizes

Standard reconstitution volumes and draw amounts for 100 mg, 250 mg, and 1 g vials.

Vial size	BAC water	Concentration	50 mg draw
100 mg	1 mL	100 mg/mL	0.5 mL (50 IU)
250 mg	2 mL	125 mg/mL	0.4 mL (40 IU)
500 mg Most common	3 mL	166.67 mg/mL	0.3 mL (30 IU)
1 g (1000 mg)	5 mL	200 mg/mL	0.25 mL (25 IU)

About NAD+ reconstitution

NAD+ (nicotinamide adenine dinucleotide) is supplied as a white lyophilized powder and dissolves readily in bacteriostatic water without aggressive mixing. Unlike many peptides, NAD+ powder tends to dissolve quickly -- gentle swirling for 30 to 60 seconds is typically sufficient. Avoid shaking, which can degrade the compound.

Research protocols commonly use subcutaneous (SubQ) administration, which requires the same reconstitution as intramuscular or intravenous routes. For SubQ, the 500 mg + 3 mL standard yields a comfortable draw volume. Common research doses range from 25 mg to 100 mg per administration, with the 50 mg reference dose used throughout this guide representing the midpoint of that range.

NAD+ is particularly sensitive to heat and light. Once reconstituted, keep the vial refrigerated at 2–8°C and wrapped in foil or stored in an opaque container. Do not freeze the reconstituted solution. Discard any vial showing discoloration, cloudiness, or visible particulates.

Last reviewed: June 2026

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Frequently asked

How much BAC water do I add to a 500 mg NAD+ vial?

For the standard ASCEND research protocol, add 3 mL of bacteriostatic water to a 500 mg NAD+ vial. This yields 166.67 mg/mL. A 50 mg dose then draws to 30 IU on a 100-unit insulin syringe.

How much BAC water do I add to a 100 mg NAD+ vial?

Add 1 mL of bacteriostatic water to a 100 mg NAD+ vial. This yields 100 mg/mL. A 50 mg dose then requires 0.5 mL (50 IU on a 100-unit syringe).

How much BAC water do I add to a 250 mg NAD+ vial?

Add 2 mL of bacteriostatic water to a 250 mg NAD+ vial. This yields 125 mg/mL. A 50 mg dose then requires 0.4 mL (40 IU on a 100-unit syringe).

How much BAC water do I add to a 1 g NAD+ vial?

Add 5 mL of bacteriostatic water to a 1 g (1000 mg) NAD+ vial. This yields 200 mg/mL. A 50 mg dose then requires 0.25 mL (25 IU on a 100-unit syringe).

What concentration does 3 mL BAC water give for 500 mg NAD+?

A 500 mg NAD+ vial reconstituted with 3 mL of bacteriostatic water yields 166.67 mg/mL. A 50 mg dose then requires 0.3 mL (30 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for NAD+?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates degradation once opened. Use sterile water only for single-use preparations.

How long is NAD+ stable once reconstituted with BAC water?

Reconstituted NAD+ stored at 2–8°C protected from light is generally stable for up to 28 days. NAD+ is more heat- and light-sensitive than many peptides -- store in a foil-wrapped or opaque container and never freeze the reconstituted solution.

How do I know if my NAD+ has fully dissolved?

The solution should be clear and colorless with no visible particulates. NAD+ typically dissolves within 30–60 seconds of gentle swirling. If any powder remains after 2 minutes, continue swirling. Discard the vial if the solution appears cloudy or discolored.

What size insulin syringe should I use for NAD+?

For a 50 mg dose from the standard 166.67 mg/mL reconstitution, draw to the 30 IU mark on a 100-unit syringe (0.3 mL). A 0.3 mL or 0.5 mL low-dead-volume syringe offers the best precision for smaller volumes.

What is the difference between NAD+ SubQ and IV reconstitution?

The reconstitution process is identical for SubQ and IV routes -- the same bacteriostatic water and concentration applies. The difference is the administration method and infusion rate, not the mixing protocol. This guide covers reconstitution only.

For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org