Recovery & Repair
GHK-Cu Dosing & Reconstitution Calculator
Copper peptide supporting wound healing, collagen synthesis, and skin rejuvenation.
TL;DR - GHK-Cu is a recovery & repair peptide with a typical starting dose of 2 mg. For a 50 mg vial, add 2 mL bacteriostatic water to yield 25 mg/mL.
2 mg
Start Dose
50 mg
Vial
2 mL
BAC Water
25
mg/mL
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Frequently Asked
How do I reconstitute GHK-Cu?
Add 2 mL of bacteriostatic water to a 50 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 25 mg/mL.
What is the recommended dose of GHK-Cu?
A typical research dose is 2 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a GHK-Cu vial?
A 50 mg vial reconstituted with 2 mL BAC water yields approximately 25 doses at 2 mg each.
How many IU is 2 mg of GHK-Cu on a syringe?
With a 50 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 25 mg/mL. For a 2 mg dose: Volume = 2 mg ÷ 25 mg/mL = 0.08 mL. Draw to the 8 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a GHK-Cu dosage calculator do?
A GHK-Cu dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (50 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the GHK-Cu reconstitution calculator formula?
Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 50 mg vial with 2 mL BAC water: concentration = 25 mg/mL. For a 2 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is the mechanism of GHK-Cu?
GHK-Cu activates wound healing by stimulating collagen and glycosaminoglycan synthesis, upregulating metalloproteinases, and modulating the TGF-beta pathway. At physiological concentrations it also has antioxidant and anti-inflammatory activity.
Can GHK-Cu be used both subcutaneously and topically?
Yes. GHK-Cu is used topically (typically 0.1–3% formulations) for skin rejuvenation and hair follicle stimulation, and subcutaneously in research for systemic anti-aging and wound-healing effects. Topical formulations require penetration enhancers for meaningful dermal absorption.
What is the half-life of GHK-Cu?
GHK-Cu has a short half-life of approximately 0.5–2 hours in plasma. Subcutaneous protocols typically use daily or twice-daily injections. Local tissue concentrations at the site of activity may persist longer than plasma measurements suggest.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for GHK-Cu?
50 mg vial reconstitution recipe + concentration table
GHK-Cu on Insulin Syringe
2 mg draw on 30u / 50u / 100u syringes
GHK-Cu + BPC-157 Stack
Wound healing + collagen synthesis
GHK-Cu + Epithalon Stack
Telomere support + copper-driven collagen
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Primary Sources
Pickart et al. Review (2015)
Collagen Synthesis (J Biomater 1996)
Data last reviewed 2026-06-03 · Methodology →
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