Recovery & Repair

GHK-Cu Dosing & Reconstitution Calculator

Copper peptide supporting wound healing, collagen synthesis, and skin rejuvenation.

TL;DR — GHK-Cu is a recovery & repair peptide with a typical starting dose of 2 mg. For a 50 mg vial, add 2 mL bacteriostatic water to yield 25 mg/mL.
2 mg
Start Dose
50 mg
Vial
2 mL
BAC Water
25
mg/mL
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Frequently Asked
How do I reconstitute GHK-Cu?
Add 2 mL of bacteriostatic water to a 50 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 25 mg/mL.
What is the recommended dose of GHK-Cu?
A typical research dose is 2 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a GHK-Cu vial?
A 50 mg vial reconstituted with 2 mL BAC water yields approximately 25 doses at 2 mg each.
Peptide Intelligence
What is the mechanism of GHK-Cu?
GHK-Cu activates wound healing by stimulating collagen and glycosaminoglycan synthesis, upregulating metalloproteinases, and modulating the TGF-beta pathway. At physiological concentrations it also has antioxidant and anti-inflammatory activity.
Can GHK-Cu be used both subcutaneously and topically?
Yes. GHK-Cu is used topically (typically 0.1–3% formulations) for skin rejuvenation and hair follicle stimulation, and subcutaneously in research for systemic anti-aging and wound-healing effects. Topical formulations require penetration enhancers for meaningful dermal absorption.
What is the half-life of GHK-Cu?
GHK-Cu has a short half-life of approximately 0.5–2 hours in plasma. Subcutaneous protocols typically use daily or twice-daily injections. Local tissue concentrations at the site of activity may persist longer than plasma measurements suggest.
Recommended Next Step
Glow Protocol
The ASCEND Glow blend (GHK-Cu + TB-500 + BPC-157) is a popular skin and tissue rejuvenation stack — all three components in one calculation.
Open Glow Blend Calculator →
Also Explore
Primary Sources
Pickart et al. Review (2015) Collagen Synthesis (J Biomater 1996)
Data last reviewed 2026-04-19 · Methodology →
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