Sexual Health
HCG Dosing & Reconstitution Calculator
Human Chorionic Gonadotropin supporting testosterone and fertility protocols.
TL;DR - HCG is a sexual health peptide with a typical starting dose of 250 IU. For a 5000 mg vial, add 2 mL bacteriostatic water to yield 2500 IU/mL.
250 IU
Start Dose
5000 mg
Vial
2 mL
BAC Water
2500
IU/mL
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Frequently Asked
How do I reconstitute HCG?
Add 2 mL of bacteriostatic water to a 5000 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2500 IU/mL.
What is the recommended dose of HCG?
A typical research dose is 250 IU. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a HCG vial?
A 5000 mg vial reconstituted with 2 mL BAC water yields approximately 20 doses at 250 IU each.
How many IU is 250 IU of HCG on a syringe?
With a 5000 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 2500 IU/mL. For a 250 IU dose: Volume = 250 IU ÷ 2500 IU/mL = 0.1 mL. Draw to the 10 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a HCG dosage calculator do?
A HCG dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (5000 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the HCG reconstitution calculator formula?
Draw volume (mL) = Dose (IU) divided by Concentration (IU/mL). For a 5000 mg vial with 2 mL BAC water: concentration = 2500 IU/mL. For a 250 IU dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is the half-life of HCG?
HCG has a half-life of approximately 24–36 hours after subcutaneous injection. Its two-phase elimination allows dosing every 2–3 days for gonadotropin protocols. This long half-life relative to LH (~20 minutes) makes it more practical for sustained testicular stimulation.
Why is HCG used in testosterone protocols?
HCG mimics LH at the Leydig cells, maintaining intratesticular testosterone production and testicular volume during exogenous testosterone use. Without HCG, exogenous testosterone suppresses LH causing testicular atrophy. Doses of 250–500 IU 2–3x/week are standard in fertility-preserving TRT protocols.
What are the storage requirements for HCG?
Lyophilized HCG powder is stable at room temperature for weeks but should be refrigerated for longer storage. Once reconstituted with bacteriostatic water, store at 2–8°C and use within 30 days. Never freeze the reconstituted solution; HCG degrades faster than many other peptides.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for HCG?
5000 mg vial reconstitution recipe + concentration table
HCG on Insulin Syringe
250 IU draw on 30u / 50u / 100u syringes
TRT Brand Reference for HCG
HCG dosing as TRT adjunct + standalone hypogonadism use
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Data last reviewed 2026-06-03 · Methodology →
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