Recovery & Repair

TB-500 Dosing & Reconstitution Calculator

Thymosin Beta-4 fragment promoting tissue repair, flexibility, and reduced inflammation.

TL;DR — TB-500 is a recovery & repair peptide with a typical starting dose of 2.5 mg. For a 5 mg vial, add 2 mL bacteriostatic water to yield 2.5 mg/mL.
2.5 mg
Start Dose
5 mg
Vial
2 mL
BAC Water
2.5
mg/mL
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Frequently Asked
How do I reconstitute TB-500?
Add 2 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2.5 mg/mL.
What is the recommended dose of TB-500?
A typical research dose is 2.5 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a TB-500 vial?
A 5 mg vial reconstituted with 2 mL BAC water yields approximately 2 doses at 2.5 mg each.
Peptide Intelligence
What is the half-life of TB-500?
TB-500 has an estimated half-life of several days when administered subcutaneously, supporting less frequent dosing such as twice weekly during loading phases. Steady-state tissue levels persist longer than plasma levels.
What does TB-500 do at a molecular level?
TB-500 sequesters G-actin, preventing actin polymerization and promoting cell migration, angiogenesis, and tissue remodeling. It upregulates metalloproteinases and downregulates inflammatory cytokines, facilitating repair in connective tissue, muscle, and heart.
Is TB-500 typically used alone or stacked with BPC-157?
TB-500 is frequently stacked with BPC-157 in repair protocols. TB-500 primarily promotes systemic tissue remodeling and angiogenesis, while BPC-157 provides strong local healing and gut-protective effects. The combination is considered synergistic by many researchers.
Recommended Next Step
Repair Stack
BPC-157 is the classic synergistic pair for TB-500 — local healing + systemic angiogenesis. Frequently stacked 1:1.
Open BPC-157 Calculator →
Also Explore
Primary Sources
Thymosin β4 Review (Ann NY Acad 2012) Cardiac Repair (Nature 2004)
Data last reviewed 2026-04-19 · Methodology →
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