Recovery & Repair
TB-500 Dosing & Reconstitution Calculator
Thymosin Beta-4 fragment promoting tissue repair, flexibility, and reduced inflammation.
TL;DR - TB-500 is a recovery & repair peptide with a typical starting dose of 2.5 mg. For a 5 mg vial, add 2 mL bacteriostatic water to yield 2.5 mg/mL.
2.5 mg
Start Dose
5 mg
Vial
2 mL
BAC Water
2.5
mg/mL
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Frequently Asked
How do I reconstitute TB-500?
Add 2 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2.5 mg/mL.
What is the recommended dose of TB-500?
A typical research dose is 2.5 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a TB-500 vial?
A 5 mg vial reconstituted with 2 mL BAC water yields approximately 2 doses at 2.5 mg each.
How many IU is 2.5 mg of TB-500 on a syringe?
With a 5 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 2.5 mg/mL. For a 2.5 mg dose: Volume = 2.5 mg ÷ 2.5 mg/mL = 1 mL. Draw to the 100 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a TB-500 dosage calculator do?
A TB-500 dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (5 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the TB-500 reconstitution calculator formula?
Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 5 mg vial with 2 mL BAC water: concentration = 2.5 mg/mL. For a 2.5 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is the half-life of TB-500?
TB-500 has an estimated half-life of several days when administered subcutaneously, supporting less frequent dosing such as twice weekly during loading phases. Steady-state tissue levels persist longer than plasma levels.
What does TB-500 do at a molecular level?
TB-500 sequesters G-actin, preventing actin polymerization and promoting cell migration, angiogenesis, and tissue remodeling. It upregulates metalloproteinases and downregulates inflammatory cytokines, facilitating repair in connective tissue, muscle, and heart.
Is TB-500 typically used alone or stacked with BPC-157?
TB-500 is frequently stacked with BPC-157 in repair protocols. TB-500 primarily promotes systemic tissue remodeling and angiogenesis, while BPC-157 provides strong local healing and gut-protective effects. The combination is considered synergistic by many researchers.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for TB-500?
5 mg vial reconstitution recipe + concentration table
TB-500 on Insulin Syringe
2.5 mg draw on 30u / 50u / 100u syringes
TB-500 + BPC-157 Stack
Classic systemic recovery stack
TB-500 + GHK-Cu Stack
Systemic regeneration + collagen synthesis
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Primary Sources
Thymosin β4 Review (Ann NY Acad 2012)
Cardiac Repair (Nature 2004)
Data last reviewed 2026-06-03 · Methodology →
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