Add 1 mL of bacteriostatic water to a 10 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 10 mg/mL.
What is the recommended dose of FOXO4-DRI?
A typical research dose is 2 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a FOXO4-DRI vial?
A 10 mg vial reconstituted with 1 mL BAC water yields approximately 5 doses at 2 mg each.
Peptide Intelligence
What is FOXO4-DRI and what makes it senolytic?
FOXO4-DRI is a D-amino acid retro-inverso peptide that disrupts the interaction between FOXO4 and p53 inside senescent cells. Senescent cells overexpress FOXO4 to sequester p53 and block apoptosis. FOXO4-DRI releases p53 from this sequestration, restoring programmed cell death selectively in senescent cells.
What is the half-life of FOXO4-DRI?
FOXO4-DRI is composed of D-amino acids in a retro-inverso configuration, making it highly resistant to proteolytic degradation. Its plasma half-life is significantly longer than equivalent L-peptides. Reported research protocols use 3-day on/4-day off cycles, suggesting multi-day biological activity.
Has FOXO4-DRI shown rejuvenation effects in animal models?
The landmark 2017 Cell paper by Baar et al. showed FOXO4-DRI selectively killed senescent cells in aged mice, improving fitness (running endurance, grip strength), restoring fur density, and improving kidney function. No significant toxicity to non-senescent cells was observed at therapeutic doses.