Longevity

Humanin Dosing & Reconstitution Calculator

Mitochondrial peptide with cytoprotective and anti-apoptotic properties.

TL;DR — Humanin is a longevity peptide with a typical starting dose of 2 mg. For a 5 mg vial, add 2 mL bacteriostatic water to yield 2.5 mg/mL.
2 mg
Start Dose
5 mg
Vial
2 mL
BAC Water
2.5
mg/mL
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Frequently Asked
How do I reconstitute Humanin?
Add 2 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2.5 mg/mL.
What is the recommended dose of Humanin?
A typical research dose is 2 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a Humanin vial?
A 5 mg vial reconstituted with 2 mL BAC water yields approximately 2 doses at 2 mg each.
Peptide Intelligence
What is Humanin and where does it originate?
Humanin is a 21-amino acid peptide encoded within the 16S rRNA gene of the mitochondrial genome, making it a mitochondria-derived peptide similar to MOTS-C. Its circulating levels decline with age and correlate inversely with insulin resistance and neurodegeneration.
What are the primary cytoprotective mechanisms of Humanin?
Humanin acts via a trimeric cytokine receptor complex and a formyl peptide receptor. It inhibits mitochondrial apoptosis pathways, protects neurons against amyloid-beta toxicity, improves insulin sensitivity, and reduces vascular inflammation.
What is the half-life of Humanin?
Humanin has a short plasma half-life of approximately 30–60 minutes, necessitating daily subcutaneous injection. Humanin-S14G (HNG) is a modified analog with approximately 1000-fold greater potency, often used at lower doses in research protocols.
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Primary Sources
Cytoprotective Peptide (PNAS 2003)
Data last reviewed 2026-04-19 · Methodology →
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