Add 1 mL of bacteriostatic water to a 1 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 1000 µg/mL.
What is the recommended dose of Klotho?
A typical research dose is 2 µg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a Klotho vial?
A 1 mg vial reconstituted with 1 mL BAC water yields approximately 500 doses at 2 µg each.
Peptide Intelligence
What is Klotho and what does it regulate?
Klotho is a longevity-associated protein expressed primarily in the kidney and brain. The circulating (soluble) form acts as a co-receptor for FGF23 signaling, regulates phosphate and calcium metabolism, suppresses oxidative stress, and modulates IGF-1 and Wnt signaling. Circulating Klotho declines significantly with age.
Why does Klotho require special handling?
Klotho is extremely fragile as a recombinant protein. Each freeze-thaw cycle can significantly reduce activity. It should be aliquoted immediately upon reconstitution into single-use volumes, stored at -80°C or -20°C for short-term, and never refrozen. Gentle handling is critical.
What cognitive effects is Klotho being researched for?
Klotho overexpression in rodents enhances synaptic plasticity and cognitive performance. A single peripheral injection of recombinant Klotho in aged mice and primates improved spatial memory without affecting IGF-1. Human genetics links higher Klotho levels to better cognition and slower neurodegeneration.