Longevity
Klotho Dosing & Reconstitution Calculator
Anti-aging protein fragment supporting kidney function and cognitive health.
TL;DR - Klotho is a longevity peptide with a typical starting dose of 2 µg. For a 1 mg vial, add 1 mL bacteriostatic water to yield 1000 µg/mL.
2 µg
Start Dose
1 mg
Vial
1 mL
BAC Water
1000
µg/mL
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Frequently Asked
How do I reconstitute Klotho?
Add 1 mL of bacteriostatic water to a 1 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 1000 µg/mL.
What is the recommended dose of Klotho?
A typical research dose is 2 µg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a Klotho vial?
A 1 mg vial reconstituted with 1 mL BAC water yields approximately 500 doses at 2 µg each.
How many IU is 2 µg of Klotho on a syringe?
With a 1 mg vial reconstituted in 1 mL bacteriostatic water, the concentration is 1000 µg/mL. For a 2 µg dose: Volume = 2 µg ÷ 1000 µg/mL = 0.002 mL. Draw to the 0.2 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a Klotho dosage calculator do?
A Klotho dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (1 mg), reconstitution volume (1 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the Klotho reconstitution calculator formula?
Draw volume (mL) = Dose (µg) divided by Concentration (µg/mL). For a 1 mg vial with 1 mL BAC water: concentration = 1000 µg/mL. For a 2 µg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is Klotho and what does it regulate?
Klotho is a longevity-associated protein expressed primarily in the kidney and brain. The circulating (soluble) form acts as a co-receptor for FGF23 signaling, regulates phosphate and calcium metabolism, suppresses oxidative stress, and modulates IGF-1 and Wnt signaling. Circulating Klotho declines significantly with age.
Why does Klotho require special handling?
Klotho is extremely fragile as a recombinant protein. Each freeze-thaw cycle can significantly reduce activity. It should be aliquoted immediately upon reconstitution into single-use volumes, stored at -80°C or -20°C for short-term, and never refrozen. Gentle handling is critical.
What cognitive effects is Klotho being researched for?
Klotho overexpression in rodents enhances synaptic plasticity and cognitive performance. A single peripheral injection of recombinant Klotho in aged mice and primates improved spatial memory without affecting IGF-1. Human genetics links higher Klotho levels to better cognition and slower neurodegeneration.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for Klotho?
1 mg vial reconstitution recipe + concentration table
Klotho on Insulin Syringe
2 µg draw on 30u / 50u / 100u syringes
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Cite This Calculator