Cognitive

P21 Dosing & Reconstitution Calculator

CNTF-derived peptide promoting neurogenesis and cognitive enhancement.

TL;DR - P21 is a cognitive peptide with a typical starting dose of 500 µg. For a 10 mg vial, add 2 mL bacteriostatic water to yield 5000 µg/mL.
500 µg
Start Dose
10 mg
Vial
2 mL
BAC Water
5000
µg/mL
500 µg draw on a 100-unit insulin syringe
010203040506070809010010 IUascendpeptide.org
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Frequently Asked
How do I reconstitute P21?
Add 2 mL of bacteriostatic water to a 10 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 5000 µg/mL.
What is the recommended dose of P21?
A typical research dose is 500 µg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a P21 vial?
A 10 mg vial reconstituted with 2 mL BAC water yields approximately 20 doses at 500 µg each.
How many IU is 500 µg of P21 on a syringe?
With a 10 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 5000 µg/mL. For a 500 µg dose: Volume = 500 µg ÷ 5000 µg/mL = 0.1 mL. Draw to the 10 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a P21 dosage calculator do?
A P21 dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (10 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the P21 reconstitution calculator formula?
Draw volume (mL) = Dose (µg) divided by Concentration (µg/mL). For a 10 mg vial with 2 mL BAC water: concentration = 5000 µg/mL. For a 500 µg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is the mechanism of P21 peptide?
P21 is derived from Ciliary Neurotrophic Factor (CNTF) and acts as a CNTF mimetic, activating JAK-STAT3 signaling to promote neurogenesis, neural progenitor cell proliferation, and synaptic plasticity. It increases hippocampal neurogenesis in rodent models.
What cognitive effects has P21 shown in research?
Rodent studies show P21 improves spatial memory, pattern separation, and reversal learning. It shows promise in models of cognitive aging and early neurodegeneration, with neurogenesis increases measurable after 4–6 weeks of administration.
What are the storage requirements for P21?
Store P21 as lyophilized powder at -20°C for long-term stability. After reconstitution, refrigerate at 2–8°C and use within 14–21 days. Avoid repeated freeze-thaw cycles. Protect from light during storage and handling.
Data last reviewed 2026-06-03 · Methodology →
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