Add 2 mL of bacteriostatic water to a 30 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 15 mg/mL.
What is the recommended dose of PNC-27?
A typical research dose is 5 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a PNC-27 vial?
A 30 mg vial reconstituted with 2 mL BAC water yields approximately 6 doses at 5 mg each.
Peptide Intelligence
What is the mechanism of PNC-27?
PNC-27 is a synthetic peptide containing a p53 MDM2-binding domain. It selectively inserts into the membranes of cells overexpressing HDM-2 (the p53 regulator), forming pores and inducing necrosis specifically in transformed cells while sparing normal cells in vitro.
What makes PNC-27 selective for abnormal cells?
Normal cells have HDM-2 intracellularly, while cells with disrupted p53 signaling overexpress membrane-associated HDM-2. PNC-27 binds this surface HDM-2, enabling selective membrane disruption of target cells without cytotoxicity to normal tissue in vitro.
What are the reconstitution and storage considerations for PNC-27?
PNC-27 requires careful reconstitution with bacteriostatic water and should be stored at -20°C as a lyophilized powder. Reconstituted solution should be used within 7–14 days when stored at 2–8°C and protected from repeated freeze-thaw cycles.