Recovery & Repair
PNC-27 Dosing & Reconstitution Calculator
Synthetic peptide that targets cells with abnormal p53 protein expression.
TL;DR - PNC-27 is a recovery & repair peptide with a typical starting dose of 5 mg. For a 30 mg vial, add 2 mL bacteriostatic water to yield 15 mg/mL.
5 mg
Start Dose
30 mg
Vial
2 mL
BAC Water
15
mg/mL
Compare Similar
Frequently Asked
How do I reconstitute PNC-27?
Add 2 mL of bacteriostatic water to a 30 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 15 mg/mL.
What is the recommended dose of PNC-27?
A typical research dose is 5 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a PNC-27 vial?
A 30 mg vial reconstituted with 2 mL BAC water yields approximately 6 doses at 5 mg each.
How many IU is 5 mg of PNC-27 on a syringe?
With a 30 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 15 mg/mL. For a 5 mg dose: Volume = 5 mg ÷ 15 mg/mL = 0.333 mL. Draw to the 33.3 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a PNC-27 dosage calculator do?
A PNC-27 dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (30 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the PNC-27 reconstitution calculator formula?
Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 30 mg vial with 2 mL BAC water: concentration = 15 mg/mL. For a 5 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is the mechanism of PNC-27?
PNC-27 is a synthetic peptide containing a p53 MDM2-binding domain. It selectively inserts into the membranes of cells overexpressing HDM-2 (the p53 regulator), forming pores and inducing necrosis specifically in transformed cells while sparing normal cells in vitro.
What makes PNC-27 selective for abnormal cells?
Normal cells have HDM-2 intracellularly, while cells with disrupted p53 signaling overexpress membrane-associated HDM-2. PNC-27 binds this surface HDM-2, enabling selective membrane disruption of target cells without cytotoxicity to normal tissue in vitro.
What are the reconstitution and storage considerations for PNC-27?
PNC-27 requires careful reconstitution with bacteriostatic water and should be stored at -20°C as a lyophilized powder. Reconstituted solution should be used within 7–14 days when stored at 2–8°C and protected from repeated freeze-thaw cycles.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for PNC-27?
30 mg vial reconstitution recipe + concentration table
PNC-27 on Insulin Syringe
5 mg draw on 30u / 50u / 100u syringes
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Data last reviewed 2026-06-03 · Methodology →
Cite This Calculator