Thymosin Beta-4 Dosing & Reconstitution Calculator

Add 2 mL of Bacteriostatic Water to a 5 mg Thymosin Beta-4 vial, injecting slowly down the glass wall. Swirl gently — never shake. This yields 2,500 mcg/mL. For a 5 mg dose, draw the full 2 mL (200 IU on a 100-unit syringe). Store at 4°C and use within 21 days.

Thymosin Beta-4 is the complete 44-amino-acid peptide (MW 4,964 Da). TB-500 is the synthetic fragment corresponding to residues 17–23 of TB-4 (Ac-LKKTETQ). TB-500 contains the actin-binding domain responsible for most of TB-4's tissue-protective effects. TB-4 additionally includes sequences involved in cardiac progenitor cell activation not present in TB-500.

TB-4's N-terminal domain (not in TB-500) activates cardiac stem cells and promotes cardiomyocyte survival after ischemic injury. Research has demonstrated TB-4 induces progenitor cell migration to damaged myocardial tissue. TB-500 is primarily studied for peripheral tissue repair; TB-4 extends into cardiac regeneration research.

A 5 mg vial reconstituted in 2 mL yields 2,500 mcg/mL. A single 5 mg dose uses the entire vial. For lower-dose loading protocols (2.5 mg), draw 1 mL (100 IU). Use the ASCEND calculator to adjust for your specific dose.