Hormonal & Fertility
Kisspeptin-54 Dosing & Reconstitution Calculator
Kisspeptin-54 — the full-length 54-amino-acid kisspeptin isoform. Potent GnRH secretagogue with longer half-life than Kisspeptin-10. Neuroendocrine fertility and HPG axis research. 1 mg vial.
TL;DR — Kisspeptin-54 is the predominant circulating kisspeptin isoform — longer half-life than KP-10 due to its extended N-terminal domain. Standard vial: 1 mg in 1 mL BAC water (1,000 mcg/mL). Research dose: 1 nmol/kg body weight. Potent GnRH pulse activator.
⚠ Note — ⚠ Dose is weight-based (1 nmol/kg). MW of KP-54 is ~6,260 Da. Use ASCEND calculator for exact mass-to-volume conversion.
1 nmol/kg
Research Dose
1 mg
Vial
1 mL
BAC Water
1,000
mcg/mL
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Frequently Asked
How do I reconstitute Kisspeptin-54?
Add 1 mL of Bacteriostatic Water to a 1 mg Kisspeptin-54 vial to yield 1,000 mcg/mL. For a 1 nmol/kg dose in a 70 kg subject (academic reference), dose = 70 × 1 nmol × 6.26 mcg/nmol = 438 mcg. Draw 0.44 mL on a 1 mL syringe. Store at 4°C; use within 7 days of reconstitution.
How does Kisspeptin-54 differ from Kisspeptin-10?
Kisspeptin-10 (KP-10) is the C-terminal 10 amino acids of kisspeptin, containing the RF-amide receptor-binding motif. Kisspeptin-54 is the full 54-amino-acid isoform with an extended N-terminal domain that confers longer plasma half-life (approximately 28 minutes for KP-54 vs 4 minutes for KP-10). KP-54 produces more sustained GnRH pulse activation.
What is the research application of Kisspeptin-54?
Kisspeptin-54 is studied for HPG axis activation in conditions of gonadotropin deficiency (hypogonadotropic hypogonadism), fertility research, and neuroendocrine signaling. It acts on KISS1R receptors on GnRH neurons, triggering GnRH release. Clinical research at NIHR Clinical Research Facility has used IV KP-54 infusions.
How does Kisspeptin-54 affect the LH pulse?
A single IV bolus of Kisspeptin-54 (1 nmol/kg in clinical research) produces a robust LH surge within 30–60 minutes via GnRH-dependent mechanisms. The amplitude and duration of LH response is greater with KP-54 than KP-10 at equimolar doses due to KP-54's longer circulating half-life.
Peptide Intelligence
What is the molecular weight of Kisspeptin-54?
Kisspeptin-54 has a molecular weight of approximately 6,260 Da. This is significantly larger than Kisspeptin-10 (1,302.5 Da), reflecting its additional 44 N-terminal residues. When calculating molar doses (nmol/kg), the mass of KP-54 per nanomole is 6.26 mcg/nmol, versus 1.30 mcg/nmol for KP-10.
What is the KISS1R receptor and where is it expressed?
KISS1R (GPR54) is a Gq-coupled receptor expressed on hypothalamic GnRH neurons. Kisspeptin binding activates phospholipase C, increasing intracellular calcium and triggering GnRH vesicle exocytosis. KISS1R is also expressed in the pituitary, gonads, and placenta, giving kisspeptin roles beyond hypothalamic GnRH gating.
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Primary Sources
Dhillo et al. — Kisspeptin-54 Human Study (2005)
Dhillo et al. — KP-54 LH Response (2007)
Data last reviewed 2026-04-20 · Methodology →
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