Add 2 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2500 µg/mL.
What is the recommended dose of PE 22-28?
A typical research dose is 400 µg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a PE 22-28 vial?
A 5 mg vial reconstituted with 2 mL BAC water yields approximately 12 doses at 400 µg each.
Peptide Intelligence
What is PE 22-28 and its mechanism of action?
PE 22-28 is a synthetic analog of spadin, derived from cleavage of NTSR3/sortilin. It activates TREK-1 (TWIK-related potassium channel 1), a mechanosensitive K+ channel linked to mood, cognition, and neuroprotection. TREK-1 activation produces antidepressant-like effects in rodent models.
What is the research dosing for PE 22-28?
PE 22-28 is in early research stages. Research protocols typically use 400–500 mcg subcutaneous doses, 1–2 times daily. Its channel-targeting mechanism differs from larger neurotrophic peptides and its pharmacokinetics in humans are not yet fully characterized.
Why is TREK-1 channel activation relevant to neurological health?
TREK-1 channels are widely expressed in the brain and spinal cord and regulate neuronal excitability, inflammatory signaling, and neuroprotection. TREK-1 knockout rodents show increased anxiety, depression, and pain sensitivity. PE 22-28's TREK-1 agonism positions it as a research tool for these conditions.