Cognitive
PE 22-28 Dosing & Reconstitution Calculator
Neuropeptide supporting myelin repair and cognitive function.
TL;DR - PE 22-28 is a cognitive peptide with a typical starting dose of 400 µg. For a 5 mg vial, add 2 mL bacteriostatic water to yield 2500 µg/mL.
400 µg
Start Dose
5 mg
Vial
2 mL
BAC Water
2500
µg/mL
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Frequently Asked
How do I reconstitute PE 22-28?
Add 2 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2500 µg/mL.
What is the recommended dose of PE 22-28?
A typical research dose is 400 µg. Consult the ASCEND calculator for your specific vial concentration and draw volume.
How many doses are in a PE 22-28 vial?
A 5 mg vial reconstituted with 2 mL BAC water yields approximately 12 doses at 400 µg each.
How many IU is 400 µg of PE 22-28 on a syringe?
With a 5 mg vial reconstituted in 2 mL bacteriostatic water, the concentration is 2500 µg/mL. For a 400 µg dose: Volume = 400 µg ÷ 2500 µg/mL = 0.16 mL. Draw to the 16 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.
What does a PE 22-28 dosage calculator do?
A PE 22-28 dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (5 mg), reconstitution volume (2 mL), and target dose to get the precise line to draw on an insulin syringe.
What is the PE 22-28 reconstitution calculator formula?
Draw volume (mL) = Dose (µg) divided by Concentration (µg/mL). For a 5 mg vial with 2 mL BAC water: concentration = 2500 µg/mL. For a 400 µg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.
Peptide Intelligence
What is PE 22-28 and its mechanism of action?
PE 22-28 is a synthetic analog of spadin, derived from cleavage of NTSR3/sortilin. It activates TREK-1 (TWIK-related potassium channel 1), a mechanosensitive K+ channel linked to mood, cognition, and neuroprotection. TREK-1 activation produces antidepressant-like effects in rodent models.
What is the research dosing for PE 22-28?
PE 22-28 is in early research stages. Research protocols typically use 400–500 mcg subcutaneous doses, 1–2 times daily. Its channel-targeting mechanism differs from larger neurotrophic peptides and its pharmacokinetics in humans are not yet fully characterized.
Why is TREK-1 channel activation relevant to neurological health?
TREK-1 channels are widely expressed in the brain and spinal cord and regulate neuronal excitability, inflammatory signaling, and neuroprotection. TREK-1 knockout rodents show increased anxiety, depression, and pain sensitivity. PE 22-28's TREK-1 agonism positions it as a research tool for these conditions.
Also Explore
Research Guides
Bacteriostatic Water Guide
What it is & why it matters for reconstitution
How Much BAC Water for PE 22-28?
5 mg vial reconstitution recipe + concentration table
PE 22-28 on Insulin Syringe
400 µg draw on 30u / 50u / 100u syringes
Insulin Syringe Guide
Choosing the right gauge & volume for your draw
Multi-Lab Verification Standard
How ASCEND ensures dosing accuracy
Data last reviewed 2026-06-03 · Methodology →
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