Hormonal & Fertility
Gonadorelin Dosing & Reconstitution Calculator
Gonadorelin (GnRH) — a decapeptide that stimulates LH and FSH release from the anterior pituitary. Used in TRT and fertility research to maintain HPG axis function. 100 mcg standard dose.
TL;DR — Gonadorelin is a synthetic GnRH decapeptide that triggers pulsatile LH and FSH release. Standard vial: 2 mg in 2 mL BAC water (1,000 mcg/mL). Typical research dose: 100 mcg twice weekly. Requires frequent dosing to mimic natural GnRH pulse frequency.
100 mcg
Start Dose
2 mg
Vial
2 mL
BAC Water
1,000
mcg/mL
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Frequently Asked
How do I reconstitute Gonadorelin?
Add 2 mL of Bacteriostatic Water to a 2 mg Gonadorelin vial, injecting slowly down the glass wall. Swirl gently — never shake. This yields 1,000 mcg/mL. A 0.1 mL draw on a 100-unit insulin syringe delivers exactly 100 mcg.
What is Gonadorelin used for in TRT research?
Gonadorelin mimics the natural pulsatile GnRH signal from the hypothalamus, stimulating the pituitary to release LH and FSH. In TRT research, it is used to maintain testicular function and HPG axis activity during testosterone supplementation protocols by preventing downstream atrophy.
How often should Gonadorelin be administered in research?
GnRH works through pulsatile signaling — continuous exposure causes receptor downregulation. Research protocols typically use 100 mcg twice weekly subcutaneously to simulate pulsatility without desensitizing GnRH receptors. More frequent dosing (daily) is also studied.
How does Gonadorelin differ from HCG?
Gonadorelin acts at the pituitary level to stimulate endogenous LH release. HCG directly mimics LH at the Leydig cell. Gonadorelin preserves the entire HPG feedback loop; HCG bypasses the pituitary. For research requiring intact neuroendocrine signaling, Gonadorelin is preferred.
Peptide Intelligence
What is the molecular weight of Gonadorelin?
Gonadorelin (pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2) has a molecular weight of 1,182.33 Da. Its decapeptide sequence is identical to endogenous GnRH. The pyroglutamate N-terminus and C-terminal amide confer resistance to aminopeptidase and carboxypeptidase degradation.
Why does continuous Gonadorelin suppress instead of stimulate?
GnRH receptors on gonadotroph cells desensitize under continuous ligand exposure through receptor internalization. This is the basis for GnRH agonist therapy in prostate cancer. Pulsatile dosing (every 90–120 minutes in vivo; twice weekly in research) maintains receptor sensitivity and gonadotropin secretion.
Also Explore
Primary Sources
Knobil — GnRH Pulse Physiology (1980)
McNeilly — Gonadotropin Regulation (2003)
Data last reviewed 2026-04-20 · Methodology →
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